Reading and Web activities

Voet: Chp8: Sect 3A

Other Sources

Crystal Structure Determination: Chp 1

In Class Activities

- Discussion of reading

2D NMR

X-ray diffraction

• Spectroscopy vs Diffraction
• Light Microscope
  • amplitude and phase
  • diffraction and refraction
  • LR ~ wavelength/2
  • size of things
• Steps
  • Growing Crystals
    • Some basic Crystallographic ideas
    • protein crystal
  • Obtaining the diffraction pattern
    • X-ray analysis of crystals
    • Experimental Setup
      • X-rays interact with electrons
      • information content of diffraction patterns
      • scattering pattern
      • intensity (I ~ |F|²) and phase
  • Model building
    • R-factor for the model
    • B-factor or temperature factor

- Molecular modeling

Go to 9. Judging the quality of models

Problems

assignment 2 exercise 3