Reading and Web activities

• Factors stabilizing the protein fold
  • disruption of stabilizing interactions
    • chemical
• Hydrogen/Deuterium Exchange
  • Example Method
  • Steps

• FTIR
  • vibrations
  • and protein structure

Read the following tutorial sections on Circular Dichroism spectroscopy

• From The Circular Dichroism Site
  • Introduction
    What is Circular Dichroism ?
    Why use Circular Dichroism ?
    • determination of the secondary structure
    • tertiary structure
    • folding kinetics
    • determine whether an expressed, purified protein is folded
    • if a mutation affects its conformation or stability.
    • protein-protein interactions
    • ligand binding
      
  • CD measurementent
    Basic experiment.
  • Protein secondary structure determination.

These will also be helpful

• Check out this Animated Tutorial (goes with What is Circular Dichroism? above)
• Article on Synchrotron Radiation CD (read the intro and nice overview of CD of proteins)
• Circular Dichroism Spectroscopy

Read the following on Fluorescence spectroscopy

• Very basic animated tutorial on fluorescence
• Similar animated tutorial on interpretation of spectra